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Et ve Peyniri Saklamak İçin Hangi Yöntem Daha İyi: Ambalaj Malzemesine Propolis Kaplamak mı, yoksa Doğrudan Gıdanın üzerine Püskürtmek mi?

Year 2024, Volume: 21 Issue: 3, 602 - 618, 27.05.2024
https://doi.org/10.33462/jotaf.1307835

Abstract

Bu çalışma, propolisin koruyucu bir kaplamasının gıda ambalaj malzemesi üzerine mi yoksa doğrudan et ve kaşar peyniri üzerine mi püskürtülmesinin daha iyi olduğunu belirlemek amacıyla gerçekleştirilmiştir. Bunu test etmek için bir vakum torbası (Poliamid/Polietilen-PA/PE), bir miktar streç film (Düşük Yoğunluklu Polietilen-LDPE) ve bir ziploc torbası (Yönlendirilmiş Polipropilen-OPP) ve gıda örneklerinin yüzeyleri propolis-etil asetat (PEA) çözeltisi püskürtülerek kaplanmıştır. Ayrıca, bu ürünlerin ambalajsız raf ömrünü incelemek için gıda yüzeylerine (peynir ve et) doğrudan propolis-propilen glikol (PPG) çözeltisi püskürtülmüştür. Paketlere yerleştirilen ve PPG çözeltisi ile kaplanan et ve kaşar peyniri sırasıyla 30 ve 45 gün boyunca 4°C'de saklanmıştır. Çalışmada kullanılan propoliste baskın fenolik bileşik 27.523,4 μg g-1 ile kafeik asit fenetil ester (CAPE), en düşük fenolik asit miktarı ise 287,53 μg g-1 ile epigallokateşin gallat olmuştur. Depolama süresinin sonunda, propolis püskürtmeli vakum ambalajda depolanan et numunesinin Enterobacteriaceae sayısı kontrole kıyasla 1,01 log CFU g-1 (p˂0,05) azalmış ve en iyi sonucu elde etmiştir. Et üzerine uygulanan PPG solüsyonunun ete iyi yapışmadığı ve solüsyonun antimikrobiyal etkisini gösteremediği sonucuna varılmıştır. Depolama süresinin sonunda, propolisle muamele edilmiş vakum torbasında tutulan kaşar peyniri örneği 21 gün boyunca en düşük TMAB sayısına sahipken (p˂0.05), kaşar peynirine doğrudan püskürtülen PPG 6.64 log CFU g-1 ile depolama sonunda en düşük TMAB sayısına sahip olmuştur (p˂0.05). PPG çözeltisi kaşar peynirinin yüzeyine yapışabilmiş ve propolis antimikrobiyal aktivitesini gösterebilmiştir. Ayrıca, propolisle muamele edilmiş vakumlu ambalajda saklanan kaşar peyniri numunesi için LAB (MRS) değeri kontrole kıyasla 0,60 log CFU g-1 (p˂0,05) azalmış ve en iyi sonucu elde etmiştir. Mikrobiyolojik analizler, vakum ambalajda propolis kaplamanın etin ve kaşar peynirinin mikrobiyolojik kalitesini iyileştirdiğini göstermiştir.

Supporting Institution

Tekirdağ Namık Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

NKUBAP.03.YL.19.225

References

  • Ali, F. H., Kassem, G. M. and Atta-Alla, O. A. (2010). La propoli come decontaminante e antiossidante nella salsiccia orientale fresca. Veterinaria Italiana, 46(2), 167–172.
  • Aliyazcoglu, R., Sahin, H., Erturk, O., Ulusoy, E. and Kolayli, S. (2013). Properties of phenolic composition and biological activity of propolis from Turkey. International Journal of Food Properties, 16(2): 277–287. https://doi.org/10.1080/10942912.2010.551312
  • Andrade, R. D., Skurtys, O. and Osorio, F. A. (2012). Atomizing spray systems for application of edible coatings. Comprehensive Reviews in Food Science and Food Safety, 11(3): 323–337. https://doi.org/10.1111/J.1541-4337.2012.00186.X
  • Apaydın, H. and Gümüş, T. (2018). Inhibitory effect of propolis (Bee Gum) against staphylococcus aureus bacteria ısolated from instant soups. Journal of Tekirdag Agricultural Faculty, 15(1): 67–75.
  • Bazargani-Gilani, B., Pajohi-Alamoti, M., Hassanzadeh, P. and Raeisi, M. (2021). Impacts of Carboxymethyl Cellulose Containing Propolis Extract on the Shelf Life of Trout Fillets. Archives of Hygiene Sciences, 10(2): 117–132. https://doi.org/10.52547/archhygsci.10.2.117
  • Bölük, E., Atik, D. S., Kolaylı, S., Demirci, A. Ş. and Palabiyik, I. (2021). Delivery of phenolics and caffeic acid phenethyl ester by propolis resin: Chewing gum system. Food Bioscience, 41:101090. https://doi.org/10.1016/j.fbio.2021.101090
  • Borges, A., Ferreira, C., Saavedra, M. J. and Simões, M. (2013). Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microbial Drug Resistance, 19(4): 256–265. https://doi.org/10.1089/mdr.2012.0244
  • Castaldo, S. and Capasso, F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73(SUPPL. 1):1–6. https://doi.org/10.1016/S0367-326X(02)00185-5
  • Chaillou, L. L. and Nazareno, M. A. (2009). Bioactivity of propolis from Santiago del Estero, Argentina, related to their chemical composition. LWT - Food Science and Technology, 42(8): 1422–1427. https://doi.org/10.1016/j.lwt.2009.03.002
  • Coban, M. Z. and Coban, O. E. (2020). Potency and use of chia mucilage coating containing propolis liquid extract for improves shelf-life of sea bass fillets. Acta Scientiarum Polonorum, Technologia Alimentaria, 19(3): 255–260. https://doi.org/10.17306/J.AFS.0843
  • Correa, F. T., de Souza, A. C., de Souza Júnior, E. A., Isidoro, S. R., Piccoli, R. H., Dias, D. R. and de Abreu, L. R. (2019). Effect of Brazilian green propolis on microorganism contaminants of surface of Gorgonzola-type cheese. Journal of Food Science and Technology, 56(4): 1978–1987. https://doi.org/10.1007/s13197-019-03664-2
  • Cushnie, T. P. T. and Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5): 343–356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
  • Dizaj, S. M., Lotfipour, F., Barzegar-Jalali, M., Zarrintan, M. H. and Adibkia, K. (2014). Antimicrobial activity of the metals and metal oxide nanoparticles. Materials Science and Engineering: C, 44: 278–284. https://doi.org/10.1016/J.MSEC.2014.08.031
  • Duman, M. and Özpolat, E. (2015). Effects of water extract of propolis on fresh shibuta (Barbus grypus) fillets during chilled storage. Food Chemistry, 189: 80–85. https://doi.org/10.1016/j.foodchem.2014.08.091
  • El-Deeb, A. and Omar, S. (2017). Effect of Propolis Extract as a Natural Preservative on the Microbial Content of Kareish Cheese. Journal of Food and Dairy Sciences, 8(7): 295–302. https://doi.org/10.21608/jfds.2017.38715
  • El-Demery, M., Elsebaie, E., Zidan, N. and Essa, R. (2016). Efficiency of Propolis and Turmeric Powders As Natural Preservatives in Minced Beef. Journal of Food and Dairy Sciences, 7(1): 45–50. https://doi.org/10.21608/jfds.2016.42805
  • Ercolini, D., Russo, F., Torrieri, E., Masi, P. and Villani, F. (2006). Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Applied and Environmental Microbiology, 72(7): 4663–4671. https://doi.org/10.1128/AEM.00468-06
  • Ertürk, Ö., Yavuz, C. and Sıralı, R. (2015). The antimicrobial activity of propolis from Ordu Province of Turkey. Mellifera, 15(1): 37–38.
  • Escriche, I. and Juan-Borrás, M. (2018). Standardizing the analysis of phenolic profile in propolis. Food Research International, 106: 834–841. https://doi.org/10.1016/j.foodres.2018.01.055
  • Fadaly H., E. and El-Badrawy, E. E. Y. (2001). Flavonoids of propolis and their antibacterial activities. Pakistan Journal of Biological Sciences, 4(2): 204–207. https://doi.org/10.3923/pjbs.2001.204.207
  • Galeotti, F., Maccari, F., Fachini, A. and Volpi, N. (2018). Chemical composition and antioxidant activity of propolis prepared in different forms and in different solvents useful for finished products. Foods, 7(3): 41. https://doi.org/10.3390/foods7030041
  • Güler, G., Şen, A., Korkmaz Turgud, F., Tahtabi̇çen, E., Ağma Okur, A. and ŞAMLI, H. E. (2022). Effects of propolis extract coating on shelf life and quality parameters of eggs obtained from free-range hens. Journal of Tekirdag Agricultural Faculty, 19(1): 89–100. https://doi.org/10.33462/JOTAF.893121
  • Güney, F. and Yılmaz, M. (2013). Propolisin kimyasal içeriği ile antibakteriyel, antiviral, antitümör, antifungal ve antioksidan aktitivitesi. Arıcılık Araştırma Dergisi, 5(1): 25–28.
  • Gutiérrez-Cortés, C. and Suarez Mahecha, H. (2014). Antimicrobial activity of propolis and its effect on the physicochemical and sensoral characteristics in sausages. Vitae, 21(2): 90–96.
  • Hames-Kocabas, E. E., Demirci, B., Uzel, A. and Demirci, F. (2013). Volatile composition of Anatolian propolis by headspace-solid-phase microextraction (HS-SPME), antimicrobial activity against food contaminants and antioxidant activity. Journal of Medicinal Plants Research, 7(28): 2140–2149. https://doi.org/10.5897/JMPR2013.4470
  • Han, S. K., Yamauchi, K. and Park, H. K. (2001). Effect of nitrite and propolis preservative on volatile basic nitrogen changes in meat products. Microbios, 105(411): 71–75.
  • Jonaidi Jafari, N., Kargozari, M., Ranjbar, R., Rostami, H. and Hamedi, H. (2018). The effect of chitosan coating incorporated with ethanolic extract of propolis on the quality of refrigerated chicken fillet. Journal of Food Processing and Preservation, 42(1): 133–136. https://doi.org/10.1111/jfpp.13336
  • Keskin, M. (2018). Alginat-Propolis mikrokapsüllerin in vitro sindirim sisteminde salınımının ham propolis ile kıyaslanması. Uludağ Arıcılık Dergisi, 18(2): 94–100. https://doi.org/10.31467/ULUARICILIK.485031
  • Kirillov, I., Asrutdinova, R., Yakupova, L., Gilmutdinov, R. and Fayzrakhmanova, G. A. (2020). Veterinary sanitary assessment of chicken meat using squalene. BIO Web of Conferences, 17(45): 00185. https://doi.org/10.1051/bioconf/20201700185
  • Labadie, J. (1999). Consequences of packaging on bacterial growth. Meat is an ecological niche. Meat Science, 52(3): 299–305. https://doi.org/10.1016/S0309-1740(99)00006-6
  • Leceta, I., Molinaro, S., Guerrero, P., Kerry, J. P. and De la Caba, K. (2015). Quality attributes of map packaged ready-to-eat baby carrots by using chitosan-based coatings. Postharvest Biology and Technology, 100: 142–150. https://doi.org/10.1016/J.POSTHARVBIO.2014.09.022
  • López-Rubio, A., Almenar, E., Hernandez-Muñoz, P., Lagarón, J. M., Catalá, R. and Gavara, R. (2004). Overview of active polymer-based packaging technologies for food applications. Food Reviews International, 20(4): 357–387. https://doi.org/10.1081/FRI-200033462
  • Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26(2): 83–99. https://doi.org/10.1051/APIDO:19950202
  • Mascheroni, E., Figoli, A., Musatti, A., Limbo, S., Drioli, E., Suevo, R., … Rollini, M. (2014). An alternative encapsulation approach for production of active chitosan-propolis beads. International Journal of Food Science and Technology, 49(5): 1401–1407. https://doi.org/10.1111/ijfs.12442
  • Mehdizadeh, T. and Mojaddar Langroodi, A. (2019). Chitosan coatings incorporated with propolis extract and Zataria multiflora Boiss oil for active packaging of chicken breast meat. International Journal of Biological Macromolecules, 141: 401–409. https://doi.org/10.1016/j.ijbiomac.2019.08.267
  • Memmedov, H., Aldemir, O. and Aliyev, E. (2017). Propolisin antioksidan ve antiinflamatuvar etkisi. Arıcılık Araştırma Dergisi, 9(2): 56–62.
  • Meng, X. H., Qin, G. Z. and Tian, S. P. (2010). Influences of preharvest spraying Cryptococcus laurentii combined with postharvest chitosan coating on postharvest diseases and quality of table grapes in storage. LWT - Food Science and Technology, 43(4): 596–601. https://doi.org/10.1016/J.LWT.2009.10.007
  • Meto, A., Colombari, B., Meto, A., Boaretto, G., Pinetti, D., Marchetti, L., … Blasi, E. (2020). Propolis affects pseudomonas aeruginosa growth, biofilm formation, eDNA release and phenazine production: Potential involvement of polyphenols. Microorganisms, 8(2): 243. https://doi.org/10.3390/microorganisms8020243
  • Metwalli, S. A. H. (2011). Extended shelf life of kareish cheese by natural preservatives. Journal of Agricultural Research, 89(2): 640–648.
  • Moawad, A., Aly, S. and Galal, E. (2001). Effect of Egyptian honeybee propolis on the growth of Aspergillus ochraceus and ochratoxin A production in Ras cheese. 8th Egyptian Conference for Dairy Science and Technology, Held at the International Agriculture Centre, Cairo, Egypt.
  • Molin, G., Ternstrom, A. and Ursing, J. (1986). Pseudomonas lundensis, a new bacterial species isolated from meat. International Journal of Systematic Bacteriology, 36(2): 339–342. https://doi.org/10.1099/00207713-36-2-339
  • Muhlisin, M., Utama, D. T., Lee, J. H., Choi, J. H. and Lee, S. K. (2016). Effects of gaseous ozone exposure on bacterial counts and oxidative properties in chicken and duck breast meat. Korean Journal for Food Science of Animal Resources, 36(3): 405–411. https://doi.org/10.5851/kosfa.2016.36.3.405
  • Nakamura, A., Takahashi, H., Otomo, K., Mizuno, Y., Kuda, T., Kimura, B. … Kobayashi, M. (2021). Dynamics of microbiota in Japanese black beef stored for a long time under chilled conditions. Food Microbiology, 100: 103849. https://doi.org/10.1016/j.fm.2021.103849
  • Narasimha Rao, D. and Sachindra, N. M. (2002). Modified atmosphere and vacuum packaging of meat and poultry products. Food Reviews International, 18(4): 263–293. https://doi.org/10.1081/FRI-120016206
  • Nessianpour, E., Khodanazary, A. and Hosseini, S. M. (2019). Shelf Life of saurida tumbil during storage at refrigeration condition as affected by gelatin-based edible coatings ıncorporated with propolis extract. International Journal of Food Properties, 22(1): 1749–1759. https://doi.org/10.1080/10942912.2019.1651738
  • Obregón Fuentes, A. M. and Rojas Hernández, N. (1990). Acción antimicrobiana de los extractos alcohólicos de propóleo. Revista Cubana de Farmacia, 24(1): 34–44.
  • Olivas, G. I. I., and Barbosa-Cánovas, G. (2009). Edible Films and Coatings For Fruits And Vegetables. In: Edible Films and Coatings for Food Applications, Eds: Huber, K. C. and Embuscado, M. E., Springer Nature. https://doi.org/10.1007/978-0-387-92824-1_7
  • Ouoba, L. I. I., Rechinger, K. B., Barkholt, V., Diawara, B., Traore, A. S. and Jakobsen, M. (2003). Degradation of proteins during the fermentation of African locust bean (Parkia biglobosa) by strains of Bacillus subtilis and Bacillus pumilus for production of Soumbala. Journal of Applied Microbiology, 94(3): 396–402. https://doi.org/10.1046/j.1365-2672.2003.01845.x
  • Payandan, E., Sayyed-Alangi, S. Z., Shamloofar, M., and Koohsari, H. (2017). Study of chemical composition and efficacy of different extracts of ıranian propolis on the microbiological and sensory parameters of minced cyprinus carpio meat at 4°C storage. Journal of Aquatic Food Product Technology, 26(5): 593–603. https://doi.org/10.1080/10498850.2016.1240281
  • Pellati, F., Orlandini, G., Pinetti, D. and Benvenuti, S. (2011). HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. Journal of Pharmaceutical and Biomedical Analysis, 55(5): 934–948. https://doi.org/10.1016/j.jpba.2011.03.024
  • Petruzzi, L., Corbo, M. R., Campaniello, D., Speranza, B., Sinigaglia, M. and Bevilacqua, A. (2020). Antifungal and antibacterial effect of propolis: A comparative hit for food-borne pseudomonas, enterobacteriaceae and fungi. Foods, 9(5): 559. https://doi.org/10.3390/foods9050559
  • Pobiega, K., Igielska, M., Włodarczyk, P. and Gniewosz, M. (2021). The use of pullulan coatings with propolis extract to extend the shelf life of blueberry (Vaccinium corymbosum) fruit. International Journal of Food Science & Technology, 56(2): 1013–1020. https://doi.org/10.1111/IJFS.14753
  • Rahman, M. M., Richardson, A. and Sofian-Azirun, M. (2010). Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 4(18): 1872–1878.
  • Ramanauskiene, K., Inkeniene, A. M., Petrikaite, V. and Briedis, V. (2013). Total phenolic content and antimicrobial activity of different lithuanian propolis solutions. Evidence-Based Complementary and Alternative Medicine, 2013: 842985. https://doi.org/10.1155/2013/842985
  • Rasul, A., Millimouno, F. M., Ali Eltayb, W., Ali, M., Li, J. and Li, X. (2013). Pinocembrin: A novel natural compound with versatile pharmacological and biological activities. BioMed Research International, 2013: 379850. https://doi.org/10.1155/2013/379850
  • Rollini, M., Mascheroni, E., Capretti, G., Coma, V., Musatti, A. and Piergiovanni, L. (2017). Propolis and chitosan as antimicrobial and polyphenols retainer for the development of paper based active packaging materials. Food Packaging and Shelf Life, 14: 75–82. https://doi.org/10.1016/j.fpsl.2017.08.011
  • Safaei, M. and Roosta Azad, R. (2020). Preparation and characterization of poly-lactic acid based films containing propolis ethanolic extract to be used in dry meat sausage packaging. Journal of Food Science and Technology, 57(4): 1242–1250. https://doi.org/10.1007/s13197-019-04156-z
  • Saleh, A., Abd El-Malek, F. and Moussa, M. (2020). Extended shelf life of tallaga cheese by natural preservatives. Journal of Productivity and Development, 25(1): 25–37. https://doi.org/10.21608/jpd.2020.81013
  • Sforcin, J. M. and Bankova, V. (2011). Propolis: Is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133(2): 253–260. https://doi.org/10.1016/j.jep.2010.10.032
  • Shavisi, N., Khanjari, A., Basti, A. A., Misaghi, A. and Shahbazi, Y. (2017). Effect of PLA films containing propolis ethanolic extract, cellulose nanoparticle and Ziziphora clinopodioides essential oil on chemical, microbial and sensory properties of minced beef. Meat Science, 124: 95–104. https://doi.org/10.1016/j.meatsci.2016.10.015 Siripatrawan, U. and Vitchayakitti, W. (2016). Improving functional properties of chitosan films as active food packaging by incorporating with propolis. Food Hydrocolloids, 61: 695–702. https://doi.org/10.1016/J.FOODHYD.2016.06.001
  • Talon, R., Lebert, I., Lebert, A., Leroy, S., Garriga, M., Aymerich, T., … Lauková, A. (2007). Traditional dry fermented sausages produced in small-scale processing units in Mediterranean countries and Slovakia. 1: Microbial ecosystems of processing environments. Meat Science, 77(4): 570–579. https://doi.org/10.1016/j.meatsci.2007.05.006
  • Tosi, B., Donini, A., Romagnoli, C. and Bruni, A. (1996). Antimicrobial activity of some commercial extracts of propolis prepared with different solvents. Phytotherapy Research, 10(4): 335–336.
  • Tucker, G. S. (2015). Food Preservation and Biodeterioration. In Food Preservation and Biodeterioration. John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118904657
  • Ustunol, Z. (2009). Edible Films and Coatings for Meat and Poultry. Edible Films and Coatings for Food Applications, Eds: Huber, K. C. and Embuscado, M. E., Springer Nature. https://doi.org/10.1007/978-0-387-92824-1_8
  • Velazquez, C., Navarro, M., Acosta, A., Angulo, A., Dominguez, Z., Robles, R., … Hernandez, J. (2007). Antibacterial and free-radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology, 103(5): 1747–1756. https://doi.org/10.1111/j.1365-2672.2007.03409.x
  • Vică, M. L., Glevitzky, M., Tit, D. M., Behl, T., Heghedűş-Mîndru, R. C., Zaha, D. C., … Bungău, S. (2021). The antimicrobial activity of honey and propolis extracts from the central region of Romania. Food Bioscience, 41: 101014. https://doi.org/10.1016/J.FBIO.2021.101014
  • Xu, H. X. and Lee, S. F. (2001). Activity of plant flavonoids against antibiotic-resistant bacteria. Phytotherapy Research, 15(1): 39–43.
  • Yingyuad, S., Ruamsin, S., Reekprkhon, D., Douglas, S., Pongamphai, S. and Siripatrawan, U. (2006). Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork. Packaging Technology and Science, 19(3): 149–157. https://doi.org/10.1002/pts.717
  • Yu, D., Jiang, Q., Xu, Y., and Xia, W. (2017). The shelf life extension of refrigerated grass carp (Ctenopharyngodon idellus) fillets by chitosan coating combined with glycerol monolaurate. International Journal of Biological Macromolecules, 101: 448–454. https://doi.org/10.1016/J.IJBIOMAC.2017.03.038
  • Yurteri, Ü. (2015). Investigation of bioactivity and chemical content of Turkish propolis from Ankara province. (MSc. Thesis) Middle East Technical University, Institute of Natural and Applied Sciences, Biotechnology Department, Ankara, Türkiye.
  • Zabaiou, N., Fouache, A., Trousson, A., Baron, S., Zellagui, A., Lahouel, M. and Lobaccaro, J. M. A. (2017). Biological properties of propolis extracts: Something new from an ancient product. Chemistry and Physics of Lipids, 207: 214–222. https://doi.org/10.1016/j.chemphyslip.2017.04.005
  • Zheng, X., Shi, X., and Wang, B. (2021). A review on the general cheese processing technology, flavor biochemical pathways and the ınfluence of yeasts in cheese. Frontiers in Microbiology, 12: 703284. https://doi.org/10.3389/FMICB.2021.703284
  • Yazgan, H., Burgut, A., Durmus, M. and Kosker, A. R. (2020). The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage. Journal of Food Safety, 40(2): 12767. https://doi.org/10.1111/jfs.12767

Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?

Year 2024, Volume: 21 Issue: 3, 602 - 618, 27.05.2024
https://doi.org/10.33462/jotaf.1307835

Abstract

This study was carried out to determine whether it is better to spray a preservative coating of propolis on the food packaging material or directly onto meat and cheddar cheese. To test this, the surfaces of a vacuum bag (Polyamide/Polyethylene-PA/PE), some stretch film (Low Density Polyethylene- LDPE) and a ziploc bag (Oriented Polypropylene-OPP) and food samples were coated by spraying them with a propolis-ethyl acetate (PEA) solution. Moreover, a propolis-propylene glycol (PPG) solution was sprayed directly on the food surfaces (cheese and meat) to study the shelf life of these products without packaging. Meat and cheddar cheese placed in packages and covered with a PPG solution were stored at 4°C for 30 and 45 days, respectively. The predominant phenolic compound in the propolis used in the study was caffeic acid phenethyl ester (CAPE) at a level of 27.523,4 μg g-1, and the lowest amount of phenolic acid was epigallocatechin gallate at 287.53 μg g-1. At the end of the storage period, the Enterobacteriaceae count of the meat sample stored in propolis sprayed vacuum packaging decreased by 1.01 log CFU g-1 (p˂0.05) compared to the control, and achieved the best result. It was concluded that the PPG solution applied onto the meat did not adhere well to it and the solution could not achieve its antimicrobial effect. At the end of the storage period, while the cheddar cheese sample kept in a propolis-treated vacuum bag had the lowest TMAB count for 21 days (p˂0.05), PPG directly sprayed on cheddar cheese had the lowest TMAB count at the end of storage with 6.64 log CFU g-1 (p˂0.05). The PPG solution was able to adhere to the surface of the cheddar cheese and propolis was able to show its antimicrobial activity. In addition, the LAB (MRS) value for the cheddar cheese sample stored in propolis-treated vacuum packaging decreased by 0.60 log CFU g-1 (p˂0.05) compared to the control and achieved the best result. Microbiological analysis showed that propolis coating in vacuum packaging improved the microbiological quality of the meat and the cheddar cheese.

Project Number

NKUBAP.03.YL.19.225

References

  • Ali, F. H., Kassem, G. M. and Atta-Alla, O. A. (2010). La propoli come decontaminante e antiossidante nella salsiccia orientale fresca. Veterinaria Italiana, 46(2), 167–172.
  • Aliyazcoglu, R., Sahin, H., Erturk, O., Ulusoy, E. and Kolayli, S. (2013). Properties of phenolic composition and biological activity of propolis from Turkey. International Journal of Food Properties, 16(2): 277–287. https://doi.org/10.1080/10942912.2010.551312
  • Andrade, R. D., Skurtys, O. and Osorio, F. A. (2012). Atomizing spray systems for application of edible coatings. Comprehensive Reviews in Food Science and Food Safety, 11(3): 323–337. https://doi.org/10.1111/J.1541-4337.2012.00186.X
  • Apaydın, H. and Gümüş, T. (2018). Inhibitory effect of propolis (Bee Gum) against staphylococcus aureus bacteria ısolated from instant soups. Journal of Tekirdag Agricultural Faculty, 15(1): 67–75.
  • Bazargani-Gilani, B., Pajohi-Alamoti, M., Hassanzadeh, P. and Raeisi, M. (2021). Impacts of Carboxymethyl Cellulose Containing Propolis Extract on the Shelf Life of Trout Fillets. Archives of Hygiene Sciences, 10(2): 117–132. https://doi.org/10.52547/archhygsci.10.2.117
  • Bölük, E., Atik, D. S., Kolaylı, S., Demirci, A. Ş. and Palabiyik, I. (2021). Delivery of phenolics and caffeic acid phenethyl ester by propolis resin: Chewing gum system. Food Bioscience, 41:101090. https://doi.org/10.1016/j.fbio.2021.101090
  • Borges, A., Ferreira, C., Saavedra, M. J. and Simões, M. (2013). Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microbial Drug Resistance, 19(4): 256–265. https://doi.org/10.1089/mdr.2012.0244
  • Castaldo, S. and Capasso, F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73(SUPPL. 1):1–6. https://doi.org/10.1016/S0367-326X(02)00185-5
  • Chaillou, L. L. and Nazareno, M. A. (2009). Bioactivity of propolis from Santiago del Estero, Argentina, related to their chemical composition. LWT - Food Science and Technology, 42(8): 1422–1427. https://doi.org/10.1016/j.lwt.2009.03.002
  • Coban, M. Z. and Coban, O. E. (2020). Potency and use of chia mucilage coating containing propolis liquid extract for improves shelf-life of sea bass fillets. Acta Scientiarum Polonorum, Technologia Alimentaria, 19(3): 255–260. https://doi.org/10.17306/J.AFS.0843
  • Correa, F. T., de Souza, A. C., de Souza Júnior, E. A., Isidoro, S. R., Piccoli, R. H., Dias, D. R. and de Abreu, L. R. (2019). Effect of Brazilian green propolis on microorganism contaminants of surface of Gorgonzola-type cheese. Journal of Food Science and Technology, 56(4): 1978–1987. https://doi.org/10.1007/s13197-019-03664-2
  • Cushnie, T. P. T. and Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5): 343–356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
  • Dizaj, S. M., Lotfipour, F., Barzegar-Jalali, M., Zarrintan, M. H. and Adibkia, K. (2014). Antimicrobial activity of the metals and metal oxide nanoparticles. Materials Science and Engineering: C, 44: 278–284. https://doi.org/10.1016/J.MSEC.2014.08.031
  • Duman, M. and Özpolat, E. (2015). Effects of water extract of propolis on fresh shibuta (Barbus grypus) fillets during chilled storage. Food Chemistry, 189: 80–85. https://doi.org/10.1016/j.foodchem.2014.08.091
  • El-Deeb, A. and Omar, S. (2017). Effect of Propolis Extract as a Natural Preservative on the Microbial Content of Kareish Cheese. Journal of Food and Dairy Sciences, 8(7): 295–302. https://doi.org/10.21608/jfds.2017.38715
  • El-Demery, M., Elsebaie, E., Zidan, N. and Essa, R. (2016). Efficiency of Propolis and Turmeric Powders As Natural Preservatives in Minced Beef. Journal of Food and Dairy Sciences, 7(1): 45–50. https://doi.org/10.21608/jfds.2016.42805
  • Ercolini, D., Russo, F., Torrieri, E., Masi, P. and Villani, F. (2006). Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Applied and Environmental Microbiology, 72(7): 4663–4671. https://doi.org/10.1128/AEM.00468-06
  • Ertürk, Ö., Yavuz, C. and Sıralı, R. (2015). The antimicrobial activity of propolis from Ordu Province of Turkey. Mellifera, 15(1): 37–38.
  • Escriche, I. and Juan-Borrás, M. (2018). Standardizing the analysis of phenolic profile in propolis. Food Research International, 106: 834–841. https://doi.org/10.1016/j.foodres.2018.01.055
  • Fadaly H., E. and El-Badrawy, E. E. Y. (2001). Flavonoids of propolis and their antibacterial activities. Pakistan Journal of Biological Sciences, 4(2): 204–207. https://doi.org/10.3923/pjbs.2001.204.207
  • Galeotti, F., Maccari, F., Fachini, A. and Volpi, N. (2018). Chemical composition and antioxidant activity of propolis prepared in different forms and in different solvents useful for finished products. Foods, 7(3): 41. https://doi.org/10.3390/foods7030041
  • Güler, G., Şen, A., Korkmaz Turgud, F., Tahtabi̇çen, E., Ağma Okur, A. and ŞAMLI, H. E. (2022). Effects of propolis extract coating on shelf life and quality parameters of eggs obtained from free-range hens. Journal of Tekirdag Agricultural Faculty, 19(1): 89–100. https://doi.org/10.33462/JOTAF.893121
  • Güney, F. and Yılmaz, M. (2013). Propolisin kimyasal içeriği ile antibakteriyel, antiviral, antitümör, antifungal ve antioksidan aktitivitesi. Arıcılık Araştırma Dergisi, 5(1): 25–28.
  • Gutiérrez-Cortés, C. and Suarez Mahecha, H. (2014). Antimicrobial activity of propolis and its effect on the physicochemical and sensoral characteristics in sausages. Vitae, 21(2): 90–96.
  • Hames-Kocabas, E. E., Demirci, B., Uzel, A. and Demirci, F. (2013). Volatile composition of Anatolian propolis by headspace-solid-phase microextraction (HS-SPME), antimicrobial activity against food contaminants and antioxidant activity. Journal of Medicinal Plants Research, 7(28): 2140–2149. https://doi.org/10.5897/JMPR2013.4470
  • Han, S. K., Yamauchi, K. and Park, H. K. (2001). Effect of nitrite and propolis preservative on volatile basic nitrogen changes in meat products. Microbios, 105(411): 71–75.
  • Jonaidi Jafari, N., Kargozari, M., Ranjbar, R., Rostami, H. and Hamedi, H. (2018). The effect of chitosan coating incorporated with ethanolic extract of propolis on the quality of refrigerated chicken fillet. Journal of Food Processing and Preservation, 42(1): 133–136. https://doi.org/10.1111/jfpp.13336
  • Keskin, M. (2018). Alginat-Propolis mikrokapsüllerin in vitro sindirim sisteminde salınımının ham propolis ile kıyaslanması. Uludağ Arıcılık Dergisi, 18(2): 94–100. https://doi.org/10.31467/ULUARICILIK.485031
  • Kirillov, I., Asrutdinova, R., Yakupova, L., Gilmutdinov, R. and Fayzrakhmanova, G. A. (2020). Veterinary sanitary assessment of chicken meat using squalene. BIO Web of Conferences, 17(45): 00185. https://doi.org/10.1051/bioconf/20201700185
  • Labadie, J. (1999). Consequences of packaging on bacterial growth. Meat is an ecological niche. Meat Science, 52(3): 299–305. https://doi.org/10.1016/S0309-1740(99)00006-6
  • Leceta, I., Molinaro, S., Guerrero, P., Kerry, J. P. and De la Caba, K. (2015). Quality attributes of map packaged ready-to-eat baby carrots by using chitosan-based coatings. Postharvest Biology and Technology, 100: 142–150. https://doi.org/10.1016/J.POSTHARVBIO.2014.09.022
  • López-Rubio, A., Almenar, E., Hernandez-Muñoz, P., Lagarón, J. M., Catalá, R. and Gavara, R. (2004). Overview of active polymer-based packaging technologies for food applications. Food Reviews International, 20(4): 357–387. https://doi.org/10.1081/FRI-200033462
  • Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26(2): 83–99. https://doi.org/10.1051/APIDO:19950202
  • Mascheroni, E., Figoli, A., Musatti, A., Limbo, S., Drioli, E., Suevo, R., … Rollini, M. (2014). An alternative encapsulation approach for production of active chitosan-propolis beads. International Journal of Food Science and Technology, 49(5): 1401–1407. https://doi.org/10.1111/ijfs.12442
  • Mehdizadeh, T. and Mojaddar Langroodi, A. (2019). Chitosan coatings incorporated with propolis extract and Zataria multiflora Boiss oil for active packaging of chicken breast meat. International Journal of Biological Macromolecules, 141: 401–409. https://doi.org/10.1016/j.ijbiomac.2019.08.267
  • Memmedov, H., Aldemir, O. and Aliyev, E. (2017). Propolisin antioksidan ve antiinflamatuvar etkisi. Arıcılık Araştırma Dergisi, 9(2): 56–62.
  • Meng, X. H., Qin, G. Z. and Tian, S. P. (2010). Influences of preharvest spraying Cryptococcus laurentii combined with postharvest chitosan coating on postharvest diseases and quality of table grapes in storage. LWT - Food Science and Technology, 43(4): 596–601. https://doi.org/10.1016/J.LWT.2009.10.007
  • Meto, A., Colombari, B., Meto, A., Boaretto, G., Pinetti, D., Marchetti, L., … Blasi, E. (2020). Propolis affects pseudomonas aeruginosa growth, biofilm formation, eDNA release and phenazine production: Potential involvement of polyphenols. Microorganisms, 8(2): 243. https://doi.org/10.3390/microorganisms8020243
  • Metwalli, S. A. H. (2011). Extended shelf life of kareish cheese by natural preservatives. Journal of Agricultural Research, 89(2): 640–648.
  • Moawad, A., Aly, S. and Galal, E. (2001). Effect of Egyptian honeybee propolis on the growth of Aspergillus ochraceus and ochratoxin A production in Ras cheese. 8th Egyptian Conference for Dairy Science and Technology, Held at the International Agriculture Centre, Cairo, Egypt.
  • Molin, G., Ternstrom, A. and Ursing, J. (1986). Pseudomonas lundensis, a new bacterial species isolated from meat. International Journal of Systematic Bacteriology, 36(2): 339–342. https://doi.org/10.1099/00207713-36-2-339
  • Muhlisin, M., Utama, D. T., Lee, J. H., Choi, J. H. and Lee, S. K. (2016). Effects of gaseous ozone exposure on bacterial counts and oxidative properties in chicken and duck breast meat. Korean Journal for Food Science of Animal Resources, 36(3): 405–411. https://doi.org/10.5851/kosfa.2016.36.3.405
  • Nakamura, A., Takahashi, H., Otomo, K., Mizuno, Y., Kuda, T., Kimura, B. … Kobayashi, M. (2021). Dynamics of microbiota in Japanese black beef stored for a long time under chilled conditions. Food Microbiology, 100: 103849. https://doi.org/10.1016/j.fm.2021.103849
  • Narasimha Rao, D. and Sachindra, N. M. (2002). Modified atmosphere and vacuum packaging of meat and poultry products. Food Reviews International, 18(4): 263–293. https://doi.org/10.1081/FRI-120016206
  • Nessianpour, E., Khodanazary, A. and Hosseini, S. M. (2019). Shelf Life of saurida tumbil during storage at refrigeration condition as affected by gelatin-based edible coatings ıncorporated with propolis extract. International Journal of Food Properties, 22(1): 1749–1759. https://doi.org/10.1080/10942912.2019.1651738
  • Obregón Fuentes, A. M. and Rojas Hernández, N. (1990). Acción antimicrobiana de los extractos alcohólicos de propóleo. Revista Cubana de Farmacia, 24(1): 34–44.
  • Olivas, G. I. I., and Barbosa-Cánovas, G. (2009). Edible Films and Coatings For Fruits And Vegetables. In: Edible Films and Coatings for Food Applications, Eds: Huber, K. C. and Embuscado, M. E., Springer Nature. https://doi.org/10.1007/978-0-387-92824-1_7
  • Ouoba, L. I. I., Rechinger, K. B., Barkholt, V., Diawara, B., Traore, A. S. and Jakobsen, M. (2003). Degradation of proteins during the fermentation of African locust bean (Parkia biglobosa) by strains of Bacillus subtilis and Bacillus pumilus for production of Soumbala. Journal of Applied Microbiology, 94(3): 396–402. https://doi.org/10.1046/j.1365-2672.2003.01845.x
  • Payandan, E., Sayyed-Alangi, S. Z., Shamloofar, M., and Koohsari, H. (2017). Study of chemical composition and efficacy of different extracts of ıranian propolis on the microbiological and sensory parameters of minced cyprinus carpio meat at 4°C storage. Journal of Aquatic Food Product Technology, 26(5): 593–603. https://doi.org/10.1080/10498850.2016.1240281
  • Pellati, F., Orlandini, G., Pinetti, D. and Benvenuti, S. (2011). HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. Journal of Pharmaceutical and Biomedical Analysis, 55(5): 934–948. https://doi.org/10.1016/j.jpba.2011.03.024
  • Petruzzi, L., Corbo, M. R., Campaniello, D., Speranza, B., Sinigaglia, M. and Bevilacqua, A. (2020). Antifungal and antibacterial effect of propolis: A comparative hit for food-borne pseudomonas, enterobacteriaceae and fungi. Foods, 9(5): 559. https://doi.org/10.3390/foods9050559
  • Pobiega, K., Igielska, M., Włodarczyk, P. and Gniewosz, M. (2021). The use of pullulan coatings with propolis extract to extend the shelf life of blueberry (Vaccinium corymbosum) fruit. International Journal of Food Science & Technology, 56(2): 1013–1020. https://doi.org/10.1111/IJFS.14753
  • Rahman, M. M., Richardson, A. and Sofian-Azirun, M. (2010). Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 4(18): 1872–1878.
  • Ramanauskiene, K., Inkeniene, A. M., Petrikaite, V. and Briedis, V. (2013). Total phenolic content and antimicrobial activity of different lithuanian propolis solutions. Evidence-Based Complementary and Alternative Medicine, 2013: 842985. https://doi.org/10.1155/2013/842985
  • Rasul, A., Millimouno, F. M., Ali Eltayb, W., Ali, M., Li, J. and Li, X. (2013). Pinocembrin: A novel natural compound with versatile pharmacological and biological activities. BioMed Research International, 2013: 379850. https://doi.org/10.1155/2013/379850
  • Rollini, M., Mascheroni, E., Capretti, G., Coma, V., Musatti, A. and Piergiovanni, L. (2017). Propolis and chitosan as antimicrobial and polyphenols retainer for the development of paper based active packaging materials. Food Packaging and Shelf Life, 14: 75–82. https://doi.org/10.1016/j.fpsl.2017.08.011
  • Safaei, M. and Roosta Azad, R. (2020). Preparation and characterization of poly-lactic acid based films containing propolis ethanolic extract to be used in dry meat sausage packaging. Journal of Food Science and Technology, 57(4): 1242–1250. https://doi.org/10.1007/s13197-019-04156-z
  • Saleh, A., Abd El-Malek, F. and Moussa, M. (2020). Extended shelf life of tallaga cheese by natural preservatives. Journal of Productivity and Development, 25(1): 25–37. https://doi.org/10.21608/jpd.2020.81013
  • Sforcin, J. M. and Bankova, V. (2011). Propolis: Is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133(2): 253–260. https://doi.org/10.1016/j.jep.2010.10.032
  • Shavisi, N., Khanjari, A., Basti, A. A., Misaghi, A. and Shahbazi, Y. (2017). Effect of PLA films containing propolis ethanolic extract, cellulose nanoparticle and Ziziphora clinopodioides essential oil on chemical, microbial and sensory properties of minced beef. Meat Science, 124: 95–104. https://doi.org/10.1016/j.meatsci.2016.10.015 Siripatrawan, U. and Vitchayakitti, W. (2016). Improving functional properties of chitosan films as active food packaging by incorporating with propolis. Food Hydrocolloids, 61: 695–702. https://doi.org/10.1016/J.FOODHYD.2016.06.001
  • Talon, R., Lebert, I., Lebert, A., Leroy, S., Garriga, M., Aymerich, T., … Lauková, A. (2007). Traditional dry fermented sausages produced in small-scale processing units in Mediterranean countries and Slovakia. 1: Microbial ecosystems of processing environments. Meat Science, 77(4): 570–579. https://doi.org/10.1016/j.meatsci.2007.05.006
  • Tosi, B., Donini, A., Romagnoli, C. and Bruni, A. (1996). Antimicrobial activity of some commercial extracts of propolis prepared with different solvents. Phytotherapy Research, 10(4): 335–336.
  • Tucker, G. S. (2015). Food Preservation and Biodeterioration. In Food Preservation and Biodeterioration. John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118904657
  • Ustunol, Z. (2009). Edible Films and Coatings for Meat and Poultry. Edible Films and Coatings for Food Applications, Eds: Huber, K. C. and Embuscado, M. E., Springer Nature. https://doi.org/10.1007/978-0-387-92824-1_8
  • Velazquez, C., Navarro, M., Acosta, A., Angulo, A., Dominguez, Z., Robles, R., … Hernandez, J. (2007). Antibacterial and free-radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology, 103(5): 1747–1756. https://doi.org/10.1111/j.1365-2672.2007.03409.x
  • Vică, M. L., Glevitzky, M., Tit, D. M., Behl, T., Heghedűş-Mîndru, R. C., Zaha, D. C., … Bungău, S. (2021). The antimicrobial activity of honey and propolis extracts from the central region of Romania. Food Bioscience, 41: 101014. https://doi.org/10.1016/J.FBIO.2021.101014
  • Xu, H. X. and Lee, S. F. (2001). Activity of plant flavonoids against antibiotic-resistant bacteria. Phytotherapy Research, 15(1): 39–43.
  • Yingyuad, S., Ruamsin, S., Reekprkhon, D., Douglas, S., Pongamphai, S. and Siripatrawan, U. (2006). Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork. Packaging Technology and Science, 19(3): 149–157. https://doi.org/10.1002/pts.717
  • Yu, D., Jiang, Q., Xu, Y., and Xia, W. (2017). The shelf life extension of refrigerated grass carp (Ctenopharyngodon idellus) fillets by chitosan coating combined with glycerol monolaurate. International Journal of Biological Macromolecules, 101: 448–454. https://doi.org/10.1016/J.IJBIOMAC.2017.03.038
  • Yurteri, Ü. (2015). Investigation of bioactivity and chemical content of Turkish propolis from Ankara province. (MSc. Thesis) Middle East Technical University, Institute of Natural and Applied Sciences, Biotechnology Department, Ankara, Türkiye.
  • Zabaiou, N., Fouache, A., Trousson, A., Baron, S., Zellagui, A., Lahouel, M. and Lobaccaro, J. M. A. (2017). Biological properties of propolis extracts: Something new from an ancient product. Chemistry and Physics of Lipids, 207: 214–222. https://doi.org/10.1016/j.chemphyslip.2017.04.005
  • Zheng, X., Shi, X., and Wang, B. (2021). A review on the general cheese processing technology, flavor biochemical pathways and the ınfluence of yeasts in cheese. Frontiers in Microbiology, 12: 703284. https://doi.org/10.3389/FMICB.2021.703284
  • Yazgan, H., Burgut, A., Durmus, M. and Kosker, A. R. (2020). The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage. Journal of Food Safety, 40(2): 12767. https://doi.org/10.1111/jfs.12767
There are 73 citations in total.

Details

Primary Language English
Subjects Food Packaging, Preservation and Processing
Journal Section Articles
Authors

İbrahim Palabıyık 0000-0001-8850-1819

Ezgi Karpuz 0000-0002-2470-3769

Project Number NKUBAP.03.YL.19.225
Early Pub Date May 21, 2024
Publication Date May 27, 2024
Submission Date May 31, 2023
Acceptance Date March 17, 2024
Published in Issue Year 2024 Volume: 21 Issue: 3

Cite

APA Palabıyık, İ., & Karpuz, E. (2024). Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?. Tekirdağ Ziraat Fakültesi Dergisi, 21(3), 602-618. https://doi.org/10.33462/jotaf.1307835
AMA Palabıyık İ, Karpuz E. Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?. JOTAF. May 2024;21(3):602-618. doi:10.33462/jotaf.1307835
Chicago Palabıyık, İbrahim, and Ezgi Karpuz. “Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?”. Tekirdağ Ziraat Fakültesi Dergisi 21, no. 3 (May 2024): 602-18. https://doi.org/10.33462/jotaf.1307835.
EndNote Palabıyık İ, Karpuz E (May 1, 2024) Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?. Tekirdağ Ziraat Fakültesi Dergisi 21 3 602–618.
IEEE İ. Palabıyık and E. Karpuz, “Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?”, JOTAF, vol. 21, no. 3, pp. 602–618, 2024, doi: 10.33462/jotaf.1307835.
ISNAD Palabıyık, İbrahim - Karpuz, Ezgi. “Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?”. Tekirdağ Ziraat Fakültesi Dergisi 21/3 (May 2024), 602-618. https://doi.org/10.33462/jotaf.1307835.
JAMA Palabıyık İ, Karpuz E. Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?. JOTAF. 2024;21:602–618.
MLA Palabıyık, İbrahim and Ezgi Karpuz. “Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?”. Tekirdağ Ziraat Fakültesi Dergisi, vol. 21, no. 3, 2024, pp. 602-18, doi:10.33462/jotaf.1307835.
Vancouver Palabıyık İ, Karpuz E. Which Method Is Better for Preserving Meat and Cheese: Coating Propolis on The Packaging Material or Spraying Directly onto The Food?. JOTAF. 2024;21(3):602-18.