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EFFECTS OF SOME HEAVY METALS (Pb++, Cu++, Cd++and Hg++) ON CYTOKININ CONTENT IN BEAN (Phaseolus vulgaris cv. Strike) SEEDLINGS

Year 2004, Volume: 2 Issue: 2, 48 - 54, 02.04.2004

Fikriye Kırbağ Zengin Ömer Munzuroğlu

Abstract

In this study, bean seedlings (Phaseolus vulgaris cv. Strike) were investigated the effects of Hg++, Cd++, Cu++ and Pb++ on cytokinin levels. Also the chlorine salts of each four heavy metals were used. Heavy metal solution prepared with pure water. The stock solution of lead was prepared at a concentration of 1.5, 2.0 and 2.5 mM (PbCl2) and other stock solution had a concentration of 0.1, 0.2 and 0.3 mM for copper (CuCl2); 0.05, 0.06, and 0.08 mM for cadmium (CdCl2.H2O); 0.02, 0.04 and 0.06mM for mercury (HgCl2). According the results we have obtained, four heavy metals caused decreases at significant rations, in the endogen cytokinin levels of seedlings. It was found that the decreases in the cytokinin content related with kind of the heavy metal and concentrations. The highest effect on cytokinin was determined in seedlings exposed to mercury. This metal followed the order Cd++ > Cu++ > Pb++. The result were applied to statistically analyze at significant levels (p<0.05 and p<0.01).

Keywords

Heavy Metal Bean Seedling Cytokinin

References

  • CABOT, C., POSCHENRIEDER, CH., BARCELO, J., “A Rapid Method for Extraction and Estimation of Abscisic Acid from Plant Tıssue Using High Performance Liquid Chromatography” Journal of Liquid Chromatography, (1986), 9(13), p 2977-2986.
  • CHEIKH, N., and JONES, R.J., “Disruption of Maize Kernel Growth and Development by Heat Stres” Plant Physiology, (1994), 106, p 45-51.
  • EL-D, A.M.S.,SALAMA, A., WARAING, P.F., “Effects of mineral Nutrition on Endogenous Cytokinins in Plants of Sunflower (Helianthus annus L.)” Journal of Experimental Botany, (1979), 30, p 971-981.
  • KENNEDY, C.D. and GONSALVES, F.A.N., “The Action of Divalent Zinc, Cadmium, Mercury, Copper and Lead on the Trans-Root Potential and Efflux of Excised Roots, Journal of Experimental Botany, (1987), 38, p 800-817.
  • KUIPER, D., SCHUIT, J. and KUIPER, P.J.C., 1989, “Efects of Internal and External
  • Cytokinin Concentrations on Root Growth and Shoot to Root Ratio of Plantago major sp. Pleiosperma at
  • Different Nutrient Conditions. 183-188 in: B.C. Loughman, O. Gasparikova, and J. Kolek (eds.), structural and functional aspects of transport in roots. Kluwer Acedemic Publishers, London.
  • LETHAM, D.S., “Regulation of Cell Division in Plant Tissuess XII a Cytokinin Bioassay Using Excisder Radish Cotyledons” Physiological Plantarum, (1971), 25, p 391-396.
  • LIDON, F.C. and HENRIQUES, F.S., “Limiting Step on Photosynthesis of Rice Plants Treated With Varying Copper Levels” Journal of Plant Physiology, (1991), 138, p 115-118.
  • LIDON, F.C., RAMALHO, J. and HENRIQUES, F.S., “Copper Inhibition of Rice Photosynthesis” Journal of. Plant Physiology, (1993), 142, p 12-17.
  • MUNZUROGLU, Ö. and GEÇKIL, H., “Effects of Metals on Seed Germination, Root Elongation, and Coleoptile and Hypocotyl Growth in Triticum aestivum and Cucumis sativus” Environmental Contamination and Toxicology, (2002), 43, p 203-213.
  • NEDELKOSKA, T.V. and DORAN, P.M. “Characteristics of Heavy Metal Uptake by Plants Species with Potential for Phytoremediation and Phytomining” Minerals Engineering, (2000), 13, p 549-561.
  • 13. OUZOUNIDOU, G., “Copper Induced Changes on Growth, Metal Content and Photosynhetic Functions of Alyssum montanum L.Plants” Envıronmental and Experımental Botany, (1994), 34, p 165-172.
  • OUZOUNIDOU G., ELEFTHERIOU, E.P. and KARATAGLIS, S., “Ecophysical and Ultrastructural Effects of Copper in Thlaspi ochroleucum” Canadian Journal of Botany, (1992), 70, p 947-957.
  • POSCHENRIEDER, C.H., GUNSE, B. and BARCELO, J., “Influence of Cadmium on Water Relations, Stomatal Resistance and Abscisic Acid Content in Expanding Bean Leaves” Plant Physiology, (1989),
  • ROVIRA, A.D., “Plant Root Excretions in Relation to the Rhizosphere Effect” Plant and Soil VII, (1956), 2, p 178-193.
  • SRESTY, T.V.S., MADHOVA RAO, K.V., “Ultrastructural Alterations in Response to Zinc and Nickel Stress in the Root Cell of Pigeonpea” Environmental and Experimental Botany, (1999), 41, p 3-13.
  • STEFFENS, J.D., “The Heavy Metal-Binding Peptides of Plants” Annual Review Plant Physiology Moleculer Biology, (1990), 41, p 533-575.
  • TAYLOR, J.S., BHALLA, M.K., ROBERTSON, J.M. and PIENING,L.J., “Cytokinins and Abscisic Acid in Hardeninig Winter Wheat” Canadian Journal of Botany, (1990), 68, p 1597-1601.
  • VAN ASSCHE, F., CEULEMANS, R. and CLIJTERS, H., “Zinc Mediated Effects on Leaf CO2 Diffusion Conductance and Net Photosynthesis in Phaseolus vulgaris L.” Photosynthesis Research, (1980), 1, p 171-180.
  • VAN STANDEN, J., COOK, E., NOODEN, L.D. (1988) “Senescence and Aging in Plants” Nooden, L.D. and Leopold, A.C.Acedemis Pres, New York 281.
  • WALKER, M.A., and DUMBROFF, E.E., “Effects of Salts Stress on Abscisic Acid and Cytokinin Levels in Tomato” Zeitschrift für Pflanzenphysiologie, (1981), 101, p 461-470.

FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ

Year 2004, Volume: 2 Issue: 2, 48 - 54, 02.04.2004

Fikriye Kırbağ Zengin Ömer Munzuroğlu

Abstract



Bu çalışmada fasulye
fidelerinin (Phaseolus vulgaris cv.
Strike) sitokinin miktarları üzerine civa, bakır, kadmiyum ve kurşunun
etkileri araştırıldı. Her dört metalinde klor tuzu kullanıldı. Ağır metal
solusyonları saf suyla hazırlandı. Civanın stok solusyonundan (HgCl2)
0.02, 0.04, 0.06 mM, kadmiyumdan (CdCl2.H2O) 0.05, 0.06
ve 0.08 mM, bakırdan (CuCl2) 0.1, 0.2 ve 0.3 mM, kurşundan (PbCl2)
ise 1.5, 2.0 ve 2.5 mM konsantrasyonları hazırlandı. Elde ettiğimiz sonuçlara
göre dört ağır metal fidelerin sitokinin seviyesinde önemli oranlarda azalışlar
meydana getirdi. Sitokinin içeriğindeki azalışın ağır metalin çeşidi ve
konsantrasyonuyla ilişkili olduğu görüldü. Fidelerin içsel sitokinin seviyesi
üzerine en fazla toksik etkiyi civa gösterdi, bu metali sırayla kadmiyum, bakır
ve kurşun izledi (Cd++ >
Cu++ > Pb++). Sonuçlar p<0.05 ve p<0.01
önem seviyelerinde istatistik analize tabi tutuldu.

Keywords

Ağır Metal Fasulye Fidesi Sitokinin

References

  • CABOT, C., POSCHENRIEDER, CH., BARCELO, J., “A Rapid Method for Extraction and Estimation of Abscisic Acid from Plant Tıssue Using High Performance Liquid Chromatography” Journal of Liquid Chromatography, (1986), 9(13), p 2977-2986.
  • CHEIKH, N., and JONES, R.J., “Disruption of Maize Kernel Growth and Development by Heat Stres” Plant Physiology, (1994), 106, p 45-51.
  • EL-D, A.M.S.,SALAMA, A., WARAING, P.F., “Effects of mineral Nutrition on Endogenous Cytokinins in Plants of Sunflower (Helianthus annus L.)” Journal of Experimental Botany, (1979), 30, p 971-981.
  • KENNEDY, C.D. and GONSALVES, F.A.N., “The Action of Divalent Zinc, Cadmium, Mercury, Copper and Lead on the Trans-Root Potential and Efflux of Excised Roots, Journal of Experimental Botany, (1987), 38, p 800-817.
  • KUIPER, D., SCHUIT, J. and KUIPER, P.J.C., 1989, “Efects of Internal and External
  • Cytokinin Concentrations on Root Growth and Shoot to Root Ratio of Plantago major sp. Pleiosperma at
  • Different Nutrient Conditions. 183-188 in: B.C. Loughman, O. Gasparikova, and J. Kolek (eds.), structural and functional aspects of transport in roots. Kluwer Acedemic Publishers, London.
  • LETHAM, D.S., “Regulation of Cell Division in Plant Tissuess XII a Cytokinin Bioassay Using Excisder Radish Cotyledons” Physiological Plantarum, (1971), 25, p 391-396.
  • LIDON, F.C. and HENRIQUES, F.S., “Limiting Step on Photosynthesis of Rice Plants Treated With Varying Copper Levels” Journal of Plant Physiology, (1991), 138, p 115-118.
  • LIDON, F.C., RAMALHO, J. and HENRIQUES, F.S., “Copper Inhibition of Rice Photosynthesis” Journal of. Plant Physiology, (1993), 142, p 12-17.
  • MUNZUROGLU, Ö. and GEÇKIL, H., “Effects of Metals on Seed Germination, Root Elongation, and Coleoptile and Hypocotyl Growth in Triticum aestivum and Cucumis sativus” Environmental Contamination and Toxicology, (2002), 43, p 203-213.
  • NEDELKOSKA, T.V. and DORAN, P.M. “Characteristics of Heavy Metal Uptake by Plants Species with Potential for Phytoremediation and Phytomining” Minerals Engineering, (2000), 13, p 549-561.
  • 13. OUZOUNIDOU, G., “Copper Induced Changes on Growth, Metal Content and Photosynhetic Functions of Alyssum montanum L.Plants” Envıronmental and Experımental Botany, (1994), 34, p 165-172.
  • OUZOUNIDOU G., ELEFTHERIOU, E.P. and KARATAGLIS, S., “Ecophysical and Ultrastructural Effects of Copper in Thlaspi ochroleucum” Canadian Journal of Botany, (1992), 70, p 947-957.
  • POSCHENRIEDER, C.H., GUNSE, B. and BARCELO, J., “Influence of Cadmium on Water Relations, Stomatal Resistance and Abscisic Acid Content in Expanding Bean Leaves” Plant Physiology, (1989),
  • ROVIRA, A.D., “Plant Root Excretions in Relation to the Rhizosphere Effect” Plant and Soil VII, (1956), 2, p 178-193.
  • SRESTY, T.V.S., MADHOVA RAO, K.V., “Ultrastructural Alterations in Response to Zinc and Nickel Stress in the Root Cell of Pigeonpea” Environmental and Experimental Botany, (1999), 41, p 3-13.
  • STEFFENS, J.D., “The Heavy Metal-Binding Peptides of Plants” Annual Review Plant Physiology Moleculer Biology, (1990), 41, p 533-575.
  • TAYLOR, J.S., BHALLA, M.K., ROBERTSON, J.M. and PIENING,L.J., “Cytokinins and Abscisic Acid in Hardeninig Winter Wheat” Canadian Journal of Botany, (1990), 68, p 1597-1601.
  • VAN ASSCHE, F., CEULEMANS, R. and CLIJTERS, H., “Zinc Mediated Effects on Leaf CO2 Diffusion Conductance and Net Photosynthesis in Phaseolus vulgaris L.” Photosynthesis Research, (1980), 1, p 171-180.
  • VAN STANDEN, J., COOK, E., NOODEN, L.D. (1988) “Senescence and Aging in Plants” Nooden, L.D. and Leopold, A.C.Acedemis Pres, New York 281.
  • WALKER, M.A., and DUMBROFF, E.E., “Effects of Salts Stress on Abscisic Acid and Cytokinin Levels in Tomato” Zeitschrift für Pflanzenphysiologie, (1981), 101, p 461-470.
There are 22 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Fikriye Kırbağ Zengin

Ömer Munzuroğlu This is me

Publication Date April 2, 2004
Published in Issue Year 2004 Volume: 2 Issue: 2

Cite

APA Kırbağ Zengin, F., & Munzuroğlu, Ö. (2004). FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ. Fırat Üniversitesi Doğu Araştırmaları Dergisi, 2(2), 48-54.
AMA Kırbağ Zengin F, Munzuroğlu Ö. FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ. (DAD). April 2004;2(2):48-54.
Chicago Kırbağ Zengin, Fikriye, and Ömer Munzuroğlu. “FASULYE FİDELERİNDEKİ (Phaseolus Vulgaris Cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ Ve Pb++) ETKİLERİ”. Fırat Üniversitesi Doğu Araştırmaları Dergisi 2, no. 2 (April 2004): 48-54.
EndNote Kırbağ Zengin F, Munzuroğlu Ö (April 1, 2004) FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ. Fırat Üniversitesi Doğu Araştırmaları Dergisi 2 2 48–54.
IEEE F. Kırbağ Zengin and Ö. Munzuroğlu, “FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ”, (DAD), vol. 2, no. 2, pp. 48–54, 2004.
ISNAD Kırbağ Zengin, Fikriye - Munzuroğlu, Ömer. “FASULYE FİDELERİNDEKİ (Phaseolus Vulgaris Cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ Ve Pb++) ETKİLERİ”. Fırat Üniversitesi Doğu Araştırmaları Dergisi 2/2 (April 2004), 48-54.
JAMA Kırbağ Zengin F, Munzuroğlu Ö. FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ. (DAD). 2004;2:48–54.
MLA Kırbağ Zengin, Fikriye and Ömer Munzuroğlu. “FASULYE FİDELERİNDEKİ (Phaseolus Vulgaris Cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ Ve Pb++) ETKİLERİ”. Fırat Üniversitesi Doğu Araştırmaları Dergisi, vol. 2, no. 2, 2004, pp. 48-54.
Vancouver Kırbağ Zengin F, Munzuroğlu Ö. FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ. (DAD). 2004;2(2):48-54.